Data/hora: 21/05/2024 19:33:18

Biblioteca(s): 	Embrapa Cerrados.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	B - 2	
Data corrente: 	16/11/2021	
Data da última atualização: 	04/04/2023	
Autoria: 	FALEIRO, F. G.; KIM, J. Y.; PARIKH, A.; ALTPETER, F.	
Afiliação: 	FABIO GELAPE FALEIRO, CPAC.	
Título: 	Generation of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Tropical Plant Biology, Sep. 2021.	
Idioma: 	Inglês	
Conteúdo: 	Napier grass (Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the production of biofuel. Genetic engineering and genome editing have tremendous potential for crop improvement and require the establishment of a transformation protocol. In this study, transgenic Napier grass was generated by introducing a minimal cassette (MC) encoding the antibiotic resistance gene nptII with biolistic gene transfer into embryogenic callus. Paromomycin was superior to geneticin as a selective agent in the selection of transgenic calli. Two high-biomass producing Napier grass accessions were successfully transformed. Southern blot analysis revealed simple transgene integration patterns with 33% of the regenerated transgenic lines having a single insert of the minimal transgene expression cassette. NPTII-ELISA confirmed transgene expression in all transgenic lines. The results of this study will enable the acceleration of Napier grass improvement by genetic transformation or genome editing.	
Thesagro: 	Capim Napier; Organismo Transgênico.	
Thesaurus Nal: 	Genetic engineering; Transgenic plants.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Cerrados (CPAC)	
Exemplares: 	CPAC - (37124-1) - UPC - AP - DD - DIGITAL -  - DIGITAL - DIS